Jump to content


- - - - -

MTT OD values: 490nm? 570nm? or 630nm?

Started by glbinbin, Oct 15 2009 04:29 PM

1 reply to this topic

#1 glbinbin

    member

  • Members
  • Pip
  • 4 posts

Posted 15 October 2009 - 04:29 PM

I used MTT assay to get known of cell proliferation. I dont know which OO values to be used. Single-wavelength 490nm? 570nm? 630nm? Or dual-wavelength?
Please give me some advice, thanks.

#2 jah

    Enthusiast

  • Active Members
  • PipPip
  • 44 posts

Posted 16 October 2009 - 04:48 AM

Formazan, the product of MTT reduction, has an absorbance at 493nm in when solubilized in DMSO, so 490nm would be your best choice from the filters you mentioned.
Back to Tissue and Cell Culture · Next Unread Topic →


  1. BioForum
  2. > Methods Discussion
  3. > Cell Biology
  4. > Tissue and Cell Culture
Home - About - Terms of Service - Privacy - Contact Us

©1999-2011 Protocol Online, All rights reserved.