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MTT OD values: 490nm? 570nm? or 630nm?


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#1 glbinbin

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Posted 15 October 2009 - 04:29 PM

I used MTT assay to get known of cell proliferation. I dont know which OO values to be used. Single-wavelength 490nm? 570nm? 630nm? Or dual-wavelength?
Please give me some advice, thanks.

#2 jah

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Posted 16 October 2009 - 04:48 AM

Formazan, the product of MTT reduction, has an absorbance at 493nm in when solubilized in DMSO, so 490nm would be your best choice from the filters you mentioned.




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