FuGene 6 "drop-wise" step
Posted 15 October 2009 - 10:41 AM
What can potentially happen if the transfection reagents were added directly into the media?
Posted 15 October 2009 - 03:40 PM
Posted 15 October 2009 - 06:57 PM
Assuming that fuGene's secret voodoo mix creates lipid-bilayered micelles:
I was thinking along similar lines, to reason it out. I was thinking that...
1) the physical shape of the droplet may further induce micelle formation
2) the droplet concentrates micelles into a foci, so a small group of cells gets a heavy dose (like a shotgun)
However, wouldn't physical force from the droplet's impact on the media's surface break existing micelles within the droplet, making it counter productive? I'm just thinking out loud here... I like to know the rationale behind techniques, please share your insights