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FuGene 6 "drop-wise" step


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#1 Bill Nye

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Posted 15 October 2009 - 10:41 AM

I'm just curious why FuGene suggests for their DNA-FuGene mix to be added in a "drop-wise" manner?
What can potentially happen if the transfection reagents were added directly into the media?

#2 bob1

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Posted 15 October 2009 - 03:40 PM

Perhaps it damages the micells in some fashion if you mix too fast, or it could cause local heating or something similar

#3 Bill Nye

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Posted 15 October 2009 - 06:57 PM

Thanks for the input bob,

Assuming that fuGene's secret voodoo mix creates lipid-bilayered micelles:
I was thinking along similar lines, to reason it out. I was thinking that...

1) the physical shape of the droplet may further induce micelle formation
2) the droplet concentrates micelles into a foci, so a small group of cells gets a heavy dose (like a shotgun)

However, wouldn't physical force from the droplet's impact on the media's surface break existing micelles within the droplet, making it counter productive? I'm just thinking out loud here... I like to know the rationale behind techniques, please share your insights :wacko:




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