Jump to content


- - - - -

PCR using genomic DNA


2 replies to this topic

#1 lufang

    member

  • Active Members
  • Pip
  • 17 posts

Posted 15 October 2009 - 07:14 AM

Anyone has some experiences on the pcr using transformed yeast genomic DNA as template. i think the transformation worked, but sometimes i can not get the band by PCR. some ideas?

#2 swanny

    Veteran

  • Active Members
  • PipPipPipPipPip
  • 536 posts

Posted 18 October 2009 - 04:56 PM

We need you protocol before we can help at all...
Be nice to your bureaucrats: they control your budgets...

#3 Prep!

    Am I me???!!!

  • Active Members
  • PipPipPipPipPip
  • 2,715 posts

Posted 22 October 2009 - 07:50 PM

i think swanny s right.. we have no information watsoever!!!
and u think it has worked?? why do you think so??
please provide some basic information for us to be able to troubleshoot!!!
Support bacteria - They are the only culture some people have!!!
Cheers!!!





Home - About - Terms of Service - Privacy - Contact Us

©1999-2011 Protocol Online, All rights reserved.