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GUS Staining Solution Calculation


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#1 Signal

Signal

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Posted 15 October 2009 - 05:09 AM

Hello,

I'm going to do GUS staining to see the promoter activity of a gene. The solution I need should be 1mM X-Gluc+50mM Phosphate buffer+10% Methanol. I doubt my calculations because I SUCK in math.

Can anybody here do this calculation for me if I need 500uL of above mentioned staining solution from stocks of 500mM X-Gluc, 50mM phosphate buffer and absolute Methanol?

Pardon me for getting into a PhD without basic math skills :P !

Thanks




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