I'm going to do GUS staining to see the promoter activity of a gene. The solution I need should be 1mM X-Gluc+50mM Phosphate buffer+10% Methanol. I doubt my calculations because I SUCK in math.
Can anybody here do this calculation for me if I need 500uL of above mentioned staining solution from stocks of 500mM X-Gluc, 50mM phosphate buffer and absolute Methanol?
Pardon me for getting into a PhD without basic math skills !
GUS Staining Solution Calculation
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