Gene B : 31.9156
well, its a good result as the standard deviation is less then 0.2
Nevertheless, on my second round of Real Time of the same assay
my result for the same sample Gene A : 13.7914 13.967 13.5537
Gene B : 31.967
You see.. for the Gene B value (CT) remain the same(reproducible) but Gene A which is a stable endogenous control varies.
Well, can you tell me why this happen? What factors affect the Ct values?
to note that my cDNA is of the same tube.
Edited by kaluts, 14 October 2009 - 06:24 PM.