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Pulse Field Gel Electrophoresis..smearing of bands


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#1 ocean_sky83

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Posted 14 October 2009 - 04:47 PM

This is a PFGE of pseudomonas aeruginosa. There is a smear at the lower molecular weight bands..Wondering any advices I can get to troubleshoot this? I am thinking it could be DNA degradation?


This is the pulse field image for pseudomonas...
[attachment=848:pfge1.JPG]

[attachment=849:pfge2.JPG]


Lane 1,8,15 are my ladders..

Any advices? Thanks!!

Edited by ocean_sky83, 14 October 2009 - 05:19 PM.






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