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viability of adherent cells


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#1 Sayeh

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Posted 14 October 2009 - 09:11 AM

Hi,
I am wondering what is the best way to measure the viability of adherent cells in culture?
Thanks

#2 bob1

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Posted 14 October 2009 - 03:10 PM

trypan blue

#3 Sayeh

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Posted 16 October 2009 - 02:04 PM

trypan blue


Thanks for the info. Would you just add trypan blue to the media? And count the cells while they are adhered?

#4 bob1

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Posted 18 October 2009 - 03:32 PM

most people would lift the cells in some fashion and then look at them in a haemocytometer. It is sort of a moot question anyway, most (I guess more than 95%) of the adherent cells will be viable - they round up and lift off when they die.




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