Hi,
I am wondering what is the best way to measure the viability of adherent cells in culture?
Thanks
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3 replies to this topic
#2
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Hmmm, I think it's working
Posted 14 October 2009 - 03:10 PM
trypan blue
#3
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Enthusiast
Posted 16 October 2009 - 02:04 PM
bob1, on Oct 14 2009, 05:10 PM, said:
trypan blue
Thanks for the info. Would you just add trypan blue to the media? And count the cells while they are adhered?
#4
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Hmmm, I think it's working
Posted 18 October 2009 - 03:32 PM
most people would lift the cells in some fashion and then look at them in a haemocytometer. It is sort of a moot question anyway, most (I guess more than 95%) of the adherent cells will be viable - they round up and lift off when they die.
