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Reprogramming


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#1 Archdupass

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Posted 13 October 2009 - 06:02 PM

Hello All,

In our lab we have quite a few Promoter driven Cre recombinase mouse lines that we often cross with RosaYFP. I was thinking of reprogramming some cells from these mice using a group of transcription factors and use the YFP as a readout. Typically in the literature a antibiotic resistance marker is used or a direct knockin cell line (Nanog-GFP). Would it be possible for a RosaYFP reporter to work?

Archdupass





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