I am trying to probe for endogenous myc which is at very low concentrations in the nucleus. I now have a band for myc, but my GAPDH is smeared on the membrane. Any suggestions for non-smeared GAPDH?
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#2
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Posted 13 October 2009 - 10:34 AM
sllwestern, on Oct 13 2009, 11:38 AM, said:
I am trying to probe for endogenous myc which is at very low concentrations in the nucleus. I now have a band for myc, but my GAPDH is smeared on the membrane. Any suggestions for non-smeared GAPDH?
What about using much less primary? or load two samples at 1x and 10x and stain for myc and GAPDH respectively.
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
#3
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Hmmm, I think it's working
Posted 13 October 2009 - 03:22 PM
Or perhaps use a shorter exposure if developing film/digital images
