Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

overloading GAPDH but nice band of interest


  • Please log in to reply
2 replies to this topic

#1 sllwestern

sllwestern

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 13 October 2009 - 07:38 AM

I am trying to probe for endogenous myc which is at very low concentrations in the nucleus. I now have a band for myc, but my GAPDH is smeared on the membrane. Any suggestions for non-smeared GAPDH?

#2 Dr Teeth

Dr Teeth

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 221 posts
1
Neutral

Posted 13 October 2009 - 10:34 AM

I am trying to probe for endogenous myc which is at very low concentrations in the nucleus. I now have a band for myc, but my GAPDH is smeared on the membrane. Any suggestions for non-smeared GAPDH?


What about using much less primary? or load two samples at 1x and 10x and stain for myc and GAPDH respectively.

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley

#3 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,826 posts
413
Excellent

Posted 13 October 2009 - 03:22 PM

Or perhaps use a shorter exposure if developing film/digital images




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.