I am trying to probe for endogenous myc which is at very low concentrations in the nucleus. I now have a band for myc, but my GAPDH is smeared on the membrane. Any suggestions for non-smeared GAPDH?
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Dr Teeth
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Posted 13 October 2009 - 10:34 AM
sllwestern, on Oct 13 2009, 11:38 AM, said:
I am trying to probe for endogenous myc which is at very low concentrations in the nucleus. I now have a band for myc, but my GAPDH is smeared on the membrane. Any suggestions for non-smeared GAPDH?
What about using much less primary? or load two samples at 1x and 10x and stain for myc and GAPDH respectively.
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bob1
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Posted 13 October 2009 - 03:22 PM
Or perhaps use a shorter exposure if developing film/digital images
