My protein is 30 kD size with pI is 4.85 determined by in silico. I rarely get single band for my protein with SDS-PAGE, as usually there are two other bands below 30 kD. But only one band appears with anti-His Western blot. When I run the OFFGEL analysis, I got three different bands with pI ranging from 4.5 to 5.0 (this could translate why there are always three bands for my protein). Does anyone know what is actually the other two proteins, is it the truncated form or the isomer of my protein?
Edited by anasyida69, 12 October 2009 - 11:54 PM.