What is the reason behind having different ligation ratios? Is it to optimize the ligation reactions?
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Posted 12 October 2009 - 09:27 AM
coolcoolz2000, on Oct 12 2009, 07:23 PM, said:
What is the reason behind having different ligation ratios? Is it to optimize the ligation reactions?
Partly yes. Since insert length/vector length ratio varies on each experiment, ligation ratio has to be determined. An optimized ratio allows to promote insert ligation inside the vector while preventing ligation of multiple inserts.
