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Different ligation ratios


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#1 coolcoolz2000

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Posted 12 October 2009 - 09:23 AM

What is the reason behind having different ligation ratios? Is it to optimize the ligation reactions?

#2 Flyingbike

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Posted 12 October 2009 - 09:27 AM

View Postcoolcoolz2000, on Oct 12 2009, 07:23 PM, said:

What is the reason behind having different ligation ratios? Is it to optimize the ligation reactions?


Partly yes. Since insert length/vector length ratio varies on each experiment, ligation ratio has to be determined. An optimized ratio allows to promote insert ligation inside the vector while preventing ligation of multiple inserts.




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