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Measuring global methylation by QPCR


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#1 ForEpi

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Posted 11 October 2009 - 11:20 PM

Hi all,

does anyone have any experience with measuring global levels of methylation using QPCR? I know of a paper by Iacopetta, et al. (2007) who uses LINE-1 and SYBRGreen. They use a methylated and unmethylated clone for their standard curve but I was wondering whether we could just use a curve using Sss1 treated and WGA DNA?

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#2 gangut

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Posted 13 October 2009 - 08:14 AM

Hi,

theoretically why not. But I guess the point is you'll never know in what % your DNA was actually methylated after SSs1 treatment, whilst having a piece of intrest cloned in a plasmid allows for sequencing it and therefore makes you sure abot the methylation status




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