Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Three bands after Ni agarosecolumn


  • Please log in to reply
1 reply to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 23 March 2001 - 10:00 PM

Dear Monica

Usually I could see the pellet with cell line RNA extraction even percipitating at RT. if not, you can add high salt precipitation solution like that provided with Trireagent ((MRC). For each 1.0 ml of Trireagent used, add to the resulting aqueous phase 0.25 ml of isopropanol followed by 0.25ml of the high salt precipitation solution (1.2M NaCl, and 0.8M Na-Citrate). High salt solution can also help remove polysaccharide and proteoglycan contamination.

Buoa fortuna!

Long



#2 Michael

Michael

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 23 March 2001 - 10:00 PM

Does anybody tell me why when I incubated acqeous phase with isopropanol at RT e centrifuge (4C) I do not see the pellet (as in Triazol method)? If I put isopropanol and acqeous phase at -20C for 1h. then I obtain the RNA pellet.Many thanksMonica




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.