Hi, does anyone know the kind of strategy to perform mutagenesis on large plasmid of around 30kb (20+kb insert) or on articifial chromosomes?
I know for large plasmids there is a XL mutagenesis kit for plasmid >8kb from strategene, but i'm not sure if it will work plasmids up to 30kb. If anyone can direct to any resources it will be great. Thanks in advance.
Doing mutagenesi for large plasmid or articificial chromosome
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