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Doing mutagenesi for large plasmid or articificial chromosome


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#1 krystle

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Posted 11 October 2009 - 11:14 PM

Hi, does anyone know the kind of strategy to perform mutagenesis on large plasmid of around 30kb (20+kb insert) or on articifial chromosomes?
I know for large plasmids there is a XL mutagenesis kit for plasmid >8kb from strategene, but i'm not sure if it will work plasmids up to 30kb. If anyone can direct to any resources it will be great. Thanks in advance.


Regards,

#2 Warren

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Posted 12 October 2009 - 07:15 PM

I would subclone a smaller fragment into a regular vector, do the mutagenesis, then subclone it back in.

Warren..




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