1 reply to this topic

[NK22]

There is a commercially avilable buffer BPERII from Piece in which I am adding Protease inhibitor cocktail III  and benzonase nuclease for the lysis of the E coli cells. But inspite of using BPERII, can I add some non ionic detergent to the Lysis Buffer(20mM Tris HCl, 5mM Imidazole, 500mM NaCl) to get the same type of cell lysis?

Edited by NK22, 11 October 2009 - 05:24 PM.

[saraarasus]

Hi,
I do the bacteria lysis with PBS and tween20+ DTT and sonication. Some time I add sarcosyl. But the lysis buffer is PBS-T. DTT or sarcosyle is for solubility. Detergent work well for me with sonication to get ride of DNA. So yes I think you can use it.



Sara

NK22, on Oct 11 2009, 05:23 PM, said:

There is a commercially avilable buffer BPERII from Piece in which I am adding Protease inhibitor cocktail III  and benzonase nuclease for the lysis of the E coli cells. But inspite of using BPERII, can I add some non ionic detergent to the Lysis Buffer(20mM Tris HCl, 5mM Imidazole, 500mM NaCl) to get the same type of cell lysis?