Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

bacteria lysis


  • Please log in to reply
1 reply to this topic

#1 NK22

NK22

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 11 October 2009 - 05:23 PM

There is a commercially avilable buffer BPERII from Piece in which I am adding Protease inhibitor cocktail III and benzonase nuclease for the lysis of the E coli cells. But inspite of using BPERII, can I add some non ionic detergent to the Lysis Buffer(20mM Tris HCl, 5mM Imidazole, 500mM NaCl) to get the same type of cell lysis?

Edited by NK22, 11 October 2009 - 05:24 PM.


#2 saraarasus

saraarasus

    member

  • Active Members
  • Pip
  • 23 posts
0
Neutral

Posted 11 October 2009 - 10:22 PM

Hi,
I do the bacteria lysis with PBS and tween20+ DTT and sonication. Some time I add sarcosyl. But the lysis buffer is PBS-T. DTT or sarcosyle is for solubility. Detergent work well for me with sonication to get ride of DNA. So yes I think you can use it.



Sara

There is a commercially avilable buffer BPERII from Piece in which I am adding Protease inhibitor cocktail III and benzonase nuclease for the lysis of the E coli cells. But inspite of using BPERII, can I add some non ionic detergent to the Lysis Buffer(20mM Tris HCl, 5mM Imidazole, 500mM NaCl) to get the same type of cell lysis?






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.