Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Intestine histology slide extraction and storage


  • Please log in to reply
1 reply to this topic

#1 cati_87

cati_87

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 10 October 2009 - 05:39 PM

Hi! I would gratly thank anyone who could give me any information about how to preserve tissues (like synovial membrane and liver slides) taken from rabbits, so as to in the future, do an RT-PCR and a Westernblot.

Thank you very much! :huh:

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,826 posts
413
Excellent

Posted 11 October 2009 - 04:00 PM

Taken fresh from rabbits? If so - snap freeze (liquid nitrogen or dry ice) in tubes and store at -80. RNA can be preserved in RNA later at room temperature, but I don't know of any similar product for protein.

If from slides - you will have problems, the fixing and mounting process cross-links proteins (that's why you do antigen retrieval) and destroys RNA by heat and formadehyde.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.