I am out of Ethanol molecular biology grade . But I have HPLC grade Ethanol. Can I use it for DNA extraction instead ?
Also in general , do you think that it's safe to use analytical grade reagents ( not Molecular biology grade ) for DNA extraction ? certainly this doesn't work for PCR reaction assembly . I just mean DNA extraction reagents e.g. Phenol , Chloroform , Ethanol , Sodium Acetate ..etc . I suppose this should not be a problem as long as DNA is finally dissolved and stored in TE buffer (Molecular Biology grade) provided the reagents aren't contaminated with nucleic acids.
Perhaps what I am thinking is not recommended for routine use But at least maybe one can use the availabe analytical grade reagent till the new molecular biology reagents supply arrive at the lab .
Have you every tried that yourself anyway ?
Edited by Bassaml7, 10 October 2009 - 05:40 AM.