1 reply to this topic

[mcb]

Has anyone experience with the miScript (Qiagen) to make cDNA of both miRs and mRNA?

They claim that it wil add an artificial polyA tail and therefore both miRNA and mRNA can be reverse transcribed. This way you should be able to run qRT-PCRs for both miRs and mRNA.

Another advantage may be that you can use mRNA as a housekeeping gene

So far we've been using the AB Taqman miRNA RT kit, which is based on stem-loop RT for specifically miRs.

Thanks!
-mcb

Edited by mcb, 09 October 2009 - 08:20 AM.

[honey]

I have used total microRNA to study mRNA expression using ABI kit it work perfectly well.