I'm culturing adherent LNCaP cells and am concerned that I have contamination - see attached pictures. I contacted HPA, who I bought the cells from, and they suggested that it could be a fault in the plastic (no) or condensation on inside of flask (also no). Does anyone have any better ideas?
Generally, the objects are round, clear looking and larger than the cells. They are floating in the media at the level of the cells (i.e. not air bubbles at the surface).
I sterile filtered all the components that I added to the base media and started fresh reagents. The cells seem to be growing fine and the media doesn't seem to be exhausting quicker than normal.
Suggestions please?
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