collagen staining in cell culture
Posted 09 October 2009 - 12:16 AM
I am looking for a procedure to stain collagen. What I have found so far does not really seem to fit my needs.
The key parameters and requirements of my setup are:
adherent fibroblast cell culture (not tissue slides)
staining the collagen secreted by the cultured cells
a procedure that does not require cell fixation (plus a non-toxic dye) for staining a living cell population would be highly welcome.
Thanks for any ideas and suggestions!
Posted 11 October 2009 - 12:38 PM
I am grateful for any advice!
Edited by verena, 11 October 2009 - 12:38 PM.
Posted 11 October 2009 - 03:53 PM
Posted 15 October 2009 - 10:32 AM
Ok, so I guess I will just have to fixate the cells...
Whats the standard method in this case?
What I mostly found was the Masson's trichrome staining, but I guess its only for histological samples, not for in vitro cell cultures.
So I think maybe antibodies are best?
Posted 21 January 2013 - 05:52 PM