Hi!
I am looking for a procedure to stain collagen. What I have found so far does not really seem to fit my needs.
The key parameters and requirements of my setup are:
adherent fibroblast cell culture (not tissue slides)
staining the collagen secreted by the cultured cells
a procedure that does not require cell fixation (plus a non-toxic dye) for staining a living cell population would be highly welcome.
Thanks for any ideas and suggestions!
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#2
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Posted 11 October 2009 - 12:38 PM
no ideas at all? 
I am grateful for any advice!
I am grateful for any advice!
Edited by verena, 11 October 2009 - 12:38 PM.
#3
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Hmmm, I think it's working
Posted 11 October 2009 - 03:53 PM
The living cell population thing is the difficult side of things - I don't know of any specific things against collagen other than antibodies, which you could try in a live environment, but probably wouldn't be very specific without the stringent washes.
#4
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Posted 15 October 2009 - 10:32 AM
Thanks for your answer!
Ok, so I guess I will just have to fixate the cells...
Whats the standard method in this case?
What I mostly found was the Masson's trichrome staining, but I guess its only for histological samples, not for in vitro cell cultures.
So I think maybe antibodies are best?
Ok, so I guess I will just have to fixate the cells...
Whats the standard method in this case?
What I mostly found was the Masson's trichrome staining, but I guess its only for histological samples, not for in vitro cell cultures.
So I think maybe antibodies are best?
