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Digestion Protocols


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#1 devil7sa

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Posted 08 October 2009 - 01:30 AM

I'm having some problems finding out a protocol to digest skin (Stratum corneum, epidermis, dermis) with enzymes!

I've determined that papain (to digest dermis - colagen) and trypsin (to digest epidermis) era the correct enzymes to the procedure, but there is nothing in the literature..I' ve looked everywhere.

Help me.

#2 molgen

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Posted 11 October 2009 - 12:25 AM

It depends on what you want to do downstream?
Culture the cells / Protein isolation / Nucleic acid isolation…




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