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T cell isolation from rat colon


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#1 bidya

bidya

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Posted 07 October 2009 - 10:05 PM

Hi all

I am isolating T cells from the colon of the Rat.
I am continuosly getting the problem of dead T cells and and very lower number of cells.
I am using percoll density centrifugation after the mincing the colon tissues.
I have also tried the clump method but the result is not at all satisfactory.

Please help me in this regard.....




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