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Freezing U937


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#1 bio01418

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Posted 07 October 2009 - 03:39 PM

Anyone share their protocol for Freezing down and thawing U937 monocytes, In the past I found them very difficult to grow from frozen.

#2 kuang

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Posted 22 October 2009 - 09:23 AM

Hi,
I am always to suspend the cell with the cryopreservation medium like 90% FBS and 10% DMSO (precool on ice for 15 min), then store in the -80 C fridge overnight. The second day transfer to liquid N2 for long-term storage.
Good luck.




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