About T-antigen and tumor cell lines
Posted 07 October 2009 - 03:31 PM
Posted 08 October 2009 - 04:59 PM
Posted 08 October 2009 - 08:33 PM
Thanks a lot. So does it mean that the plasmids which only contain SV-40 ori can't replicate themselves in these cell lines which not transformed by SV-40?
No not all cells have T-antigen, only those transformed by SV-40. There are other similar proteins expressed by other viruses that work equally well.