2 replies to this topic

[bio01418]

I heared that  T-antigen was expressed in the early phase of transforming cells. So do anyone know that if all tumor cell lines express T-antigen? and what about NIH/3T3 cell line?

[bob1]

No not all cells have T-antigen, only those transformed by SV-40.  There are other similar proteins expressed by other viruses that work equally well.

[bio01418]

bob1, on Oct 8 2009, 04:59 PM, said:

No not all cells have T-antigen, only those transformed by SV-40.  There are other similar proteins expressed by other viruses that work equally well.

Thanks a lot. So does it mean that the plasmids which only contain SV-40 ori can't replicate themselves in these cell lines  which not transformed by SV-40?