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About T-antigen and tumor cell lines


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#1 bio01418

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Posted 07 October 2009 - 03:31 PM

I heared that T-antigen was expressed in the early phase of transforming cells. So do anyone know that if all tumor cell lines express T-antigen? and what about NIH/3T3 cell line?

#2 bob1

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Posted 08 October 2009 - 04:59 PM

No not all cells have T-antigen, only those transformed by SV-40. There are other similar proteins expressed by other viruses that work equally well.

#3 bio01418

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Posted 08 October 2009 - 08:33 PM

No not all cells have T-antigen, only those transformed by SV-40. There are other similar proteins expressed by other viruses that work equally well.

Thanks a lot. So does it mean that the plasmids which only contain SV-40 ori can't replicate themselves in these cell lines which not transformed by SV-40?




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