RNA from whole blood
Posted 05 July 2001 - 09:00 PM
Posted 25 October 2001 - 09:00 PM
Posted 03 June 2012 - 01:42 PM
Posted 04 June 2012 - 04:12 AM
Just last week I tried the Tri-reagent to extract RNA from PBMCs containing virus in it and got no RNA. we usually use Trireagent for RNA extraction and gives very high yiled. But this one was my first trial with PBMCs and I don`t know what went wrong?
Posted 04 June 2012 - 04:15 AM
Sigma sells a trireagent specially for blood.
I would not suggest to do the RNA extraction from whole blood unless it is necessary. If so you can still use trireagent but as far as I know because of potential contamination risk you would have to do some sort of purification step afterwards.
Posted 04 June 2012 - 10:14 PM
Edited by AquaPlasmid, 04 June 2012 - 10:15 PM.