Help! Plasmid disappearing after mini-prep.
Posted 07 October 2009 - 02:24 PM
Posted 08 October 2009 - 08:15 AM
Posted 13 October 2009 - 04:27 AM
1. contamination, no plasmid . might wanna smell ur culture and see if it got the E.coli scent
2. maybe something wrong with ur plasmid prep. A260 could be from RNA as well, but somehow i feel it as quite unlikely as RNAse is normally included in most prep
How was ur minipreps done? this is quite hard to determine. the gel part seems fine. control was there. no dye floating out i assume (etoh contamination ) when loading?