Posted 07 October 2009 - 12:08 PM
I am trying to stablily knockdown a gene in our cancer cell line. I found that Santa cruz is sellling all kinds of shRNA plasmids and lentiviral particles, so do some other companys. Has anyone used their similar products and could you give me some suggestion which company is more reliable?
Posted 10 October 2009 - 08:01 AM
Invitrogen: BLOCK-iT system
System Biosciences: pSIH1-H1 vectors
Biosettia: pLV-RNAi vectors
Open Biosystem: pTRIPZ system
Addgene: pLKO.1 and pLL3.7
and many others..............
My experience is: Invitrogen's shRNA designer seems the best and Biosettia's lentiviral shRNA vector only needs one shRNA oligo (single oligonucleotide RNAi technology) for each shRNA clone you make (save you 15 dollars per oligo sysnthesis).
Posted 13 October 2009 - 04:05 AM
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley