I want to trnsfect the Jurkat cells (Lymphoblastoid cell line) and EL-4 . and I have tried the calcium phosphate method for the same but there is very less efficiency. please let me know any other method other than electroporation for transfecting these cells.
0
2 replies to this topic
#1
anonymous
-
-
- 1,890 posts
Veteran
0
Neutral
Neutral
Posted 20 July 2001 - 09:00 PM
Hi Friends
#3
jzhen
-
- Members
-
- 3 posts
member
0
Neutral
Neutral
Posted 08 April 2004 - 10:50 AM
[COLOR=purple] I am starting to do some transfection. I am using lipofectamine, which I think is a kind of cationic polymers. I am using Lipofectamine. It is said to have high effeciency. Also, I have some questions to ask too. I understand why I could not use antibiotics, such as G418, in the medium for transfection. But how about penicillin/ streptomycin? Can I use P/S as later maintaince the cell line?
Thanks!
Juan
[FONT=Arial][[COLOR=purple]
