Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

transfection


  • Please log in to reply
2 replies to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 20 July 2001 - 09:00 PM

Hi Friends

I want to trnsfect the Jurkat cells (Lymphoblastoid cell line) and EL-4 . and I have tried the calcium phosphate method for the same but there is very less efficiency. please let me know any other method other than electroporation for transfecting these cells.


#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 29 July 2001 - 09:00 PM

have you tried liposomes?

#3 jzhen

jzhen

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 08 April 2004 - 10:50 AM

:angry: [COLOR=purple] I am starting to do some transfection. I am using lipofectamine, which I think is a kind of cationic polymers. I am using Lipofectamine. It is said to have high effeciency.
Also, I have some questions to ask too. I understand why I could not use antibiotics, such as G418, in the medium for transfection. But how about penicillin/ streptomycin? Can I use P/S as later maintaince the cell line?
Thanks!
Juan
[FONT=Arial][[COLOR=purple]




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.