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Change in pH after adding Genticin to the media


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#1 cell_man

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Posted 07 October 2009 - 08:25 AM

Hi,

I am making stock solutions of neomycin ranging from 200ug/mL to 2mg/mL to perform a kill curve experiment. But after adding neomycin to the medium, the color is changing to yellow, particualrly in the ones with higher neomycin concentration. Is this media good for the culture of my 293 cells or should I readjust the pH? if yes, how? Do you encounter the same situation or am i doing it wrong? As you can see I am kind of new to cell culture.

Thanks in advance <_<

#2 Dr Teeth

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Posted 07 October 2009 - 09:38 AM

Hi,

I am making stock solutions of neomycin ranging from 200ug/mL to 2mg/mL to perform a kill curve experiment. But after adding neomycin to the medium, the color is changing to yellow, particualrly in the ones with higher neomycin concentration. Is this media good for the culture of my 293 cells or should I readjust the pH? if yes, how? Do you encounter the same situation or am i doing it wrong? As you can see I am kind of new to cell culture.

Thanks in advance :(



I readjust the pH with 0.2 um filtered 0.1 M NaOH directly to the medium bottle after adding neomycin.

Edited by Dr Teeth, 07 October 2009 - 09:39 AM.


Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley

#3 cell_man

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Posted 07 October 2009 - 11:05 AM

I readjust the pH with 0.2 um filtered 0.1 M NaOH directly to the medium bottle after adding neomycin.

Thanks a lot Dr.Teeth, I will try that.

#4 porfirion

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Posted 08 October 2009 - 10:53 PM

you can also add sodium bicarbonate (NaHCO3) itīs harmful even in high dosis and many media contain it eg DMEM




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