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Cycloheximide Concentration


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#1 mattbat7

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Posted 07 October 2009 - 07:23 AM

Hi,

I'm trying to inhibit a stock strain of wild-type Saccharomyces with cycloheximide.....but am unsure what concentration to use.


Does anybody know of a resource i can use, or what concentration is commonly used?

Thanks

Mattbat

#2 GeorgeWolff

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Posted 07 October 2009 - 01:38 PM

Matt - the levels I've used are ~ 0.5g/liter.

#3 GeorgeWolff

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Posted 07 October 2009 - 03:31 PM

Saw (old) paper (http://jb.asm.org/cg...stract/86/5/956 ) reporting apparent cycloheximide induction of petite variant of related species.




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