2 replies to this topic

[mattbat7]

Hi,

I'm trying to inhibit a stock strain of wild-type Saccharomyces with cycloheximide.....but am unsure what concentration to use.


Does anybody know of a resource i can use, or what concentration is commonly used?

Thanks

Mattbat

[GeorgeWolff]

Matt - the levels I've used are ~ 0.5g/liter.

[GeorgeWolff]

Saw (old) paper (http://jb.asm.org/cg...stract/86/5/956 ) reporting apparent cycloheximide induction of petite variant of related species.