Thank you, do you mean coat the plates directly with the antibody instead of using the streptavidin biotin system?
We tried that originally with maxisorb plates, and had varying results (maybe due to the unknown factor that has now disappeared). We could try that again now that the random noise seems to have gone and see whether it gives us a lower background noise.
I'll let you know how our blocking experiments go. My colleague has gone for a Pierce product Protein Free Blocker - I'll let you know how that goes too.
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Problem with ELISA false positives and noise
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