Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Problem with ELISA false positives and noise

  • Please log in to reply
15 replies to this topic

#16 AndreaELISA



  • Active Members
  • Pip
  • 8 posts

Posted 30 October 2009 - 12:26 PM

Thank you, do you mean coat the plates directly with the antibody instead of using the streptavidin biotin system?

We tried that originally with maxisorb plates, and had varying results (maybe due to the unknown factor that has now disappeared). We could try that again now that the random noise seems to have gone and see whether it gives us a lower background noise.

I'll let you know how our blocking experiments go. My colleague has gone for a Pierce product Protein Free Blocker - I'll let you know how that goes too.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.