Could I denature the proteins that were transferred on a membrane?
Posted 07 October 2009 - 01:13 AM
Posted 07 October 2009 - 05:08 AM
Posted 08 October 2009 - 02:33 AM
Today I tried a high stringent condion. Wasing the membrane with 8 M urea-PBST plus 10% beta-ME for 1 hr, the result is no signal detected. It seems that all the proteins shed away.
Maybe it is impossible to do that. To denature proteins may also disrupt the binding between proteins and a membrane.
I will look for another antibody working well on native samples.
Posted 08 October 2009 - 06:52 AM