Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Expression in yeast


  • Please log in to reply
No replies to this topic

#1 paramyosin

paramyosin

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 34 posts
0
Neutral

Posted 06 October 2009 - 11:54 PM

Hello !!
I want to express a viral gene in Pichia pastoris but, this is my first time......any of you have experienece in this issue?, i would like to know if saccharomyces is more convenient or in the case of Pichia which plasmid is the best one. I have read something about pPICZ, pPIC6, pGAPZ. Apparently all of them are terrific (for Invitrogen) but...there are always unofficial hints that can be obtainded from expert hands. If you have any suggestion about a different expresion plasmid for Pichia, please let me know, i donīt want to make the blunder of the beginer.

Thank you in advance!!!!
"Research without indebtedness is suspect, and somebody must always, somehow, be thanked." Umberto Eco




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.