I want to express a viral gene in Pichia pastoris but, this is my first time......any of you have experienece in this issue?, i would like to know if saccharomyces is more convenient or in the case of Pichia which plasmid is the best one. I have read something about pPICZ, pPIC6, pGAPZ. Apparently all of them are terrific (for Invitrogen) but...there are always unofficial hints that can be obtainded from expert hands. If you have any suggestion about a different expresion plasmid for Pichia, please let me know, i donīt want to make the blunder of the beginer.
Thank you in advance!!!!
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Expression in yeast
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