Cell-cycle independant qRT-PCR internal control

Started by Sean18, Oct 06 2009 08:40 PM

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#1 Sean18

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Posted 06 October 2009 - 08:40 PM

Hi,

I am currently working on qRT-PCR, studying differential gene expression of human cells from diferent cell cycles. I am wondering which internal control should i use? as normal housekeeping genes such as GAPDH, b-actin may vary with different cell cycle.

Has anyone come across this?any suggestions of genes to be used in this case? (cell-cycle independant -internal control)

Sean