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Cell-cycle independant qRT-PCR internal control


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#1 Sean18

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Posted 06 October 2009 - 08:40 PM

Hi,

I am currently working on qRT-PCR, studying differential gene expression of human cells from diferent cell cycles. I am wondering which internal control should i use? as normal housekeeping genes such as GAPDH, b-actin may vary with different cell cycle.

Has anyone come across this?any suggestions of genes to be used in this case? (cell-cycle independant -internal control)

Sean

#2 sandi

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Posted 07 October 2009 - 01:57 AM

how about GUS?




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