I am currently working on qRT-PCR, studying differential gene expression of human cells from diferent cell cycles. I am wondering which internal control should i use? as normal housekeeping genes such as GAPDH, b-actin may vary with different cell cycle.
Has anyone come across this?any suggestions of genes to be used in this case? (cell-cycle independant -internal control)
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Cell-cycle independant qRT-PCR internal control
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