Posted 06 October 2009 - 07:11 PM
I'd like to determine in SDS-PAGE the relative proportion of my purified recombinant protein.
Is there a software which I can check if my purified protein represent, for exemple, 90% of the protein content of the elution fraction?
Is there other ways to check the purity of my purification?
Thanks in advance.
Posted 06 October 2009 - 10:32 PM
Posted 24 October 2009 - 02:00 AM
if a qualitative result is fine for you, try running a 10% solution of the pure protein and compare the intensity of the impurities of your fraction with this 10% and tat will give u a qualitative estimate!!!