reconstitution of lyophilized enzyme (soybean 15-lipoxygenase-1, Sigma L7395), how to?
ive tried using sodium borate buffer (.2M pH 9) but it doesnt fully dissolve in it. any suggestions?
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#2
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Veteran
Posted 06 October 2009 - 09:55 PM
Does Sigma have any suggestions? Try the technical specialists in your area.
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#3
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an elder
Posted 07 October 2009 - 10:27 AM
the information sheet says that it is soluble to 0.1mg/ml. are you trying to make it too concentrated?
it may take a little time to fully solubilize.
it may take a little time to fully solubilize.
Edited by mdfenko, 07 October 2009 - 10:28 AM.
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#4
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member
Posted 18 November 2009 - 10:23 PM
Solubility is usually a matter of pH, salt and something else (glycerol, BME, EDTA etc.).
Got to play around with these factors and identify the sweet spot (or look it up in a publication that goes into a description of the buffer).
My 2 ct
Dave
Protein Solubility kits
Got to play around with these factors and identify the sweet spot (or look it up in a publication that goes into a description of the buffer).
My 2 ct
Dave
Protein Solubility kits
