Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

how to distinguish between LOH and aUPD?


  • Please log in to reply
1 reply to this topic

#1 Lala

Lala

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 06 October 2009 - 02:59 PM

Hi, I would really appreciate it, if someone could point me in the right direction on how to answer the following question.

question:

How would you distinguish between LOH and aUPD by way of using microsatellite analysis and MLPA.
are there any textbooks or journal articles I can read to find this out? i did some searches but couldnt find anything :(

this is what i know so far:

aUPD is Uniparental disomy - ocurs when a person inherits two copies of a chromosome pair from one parent and no copy from the other parent. but normally a baby will inherit one copy of each chromosome from the mother and father. so aUPD is when having the SAME copy of the chromosome. a form of LOH

but what does the a stand for in aUPD ?

also i know that you can use microsatellite markers to tell when LOH has occured as you will see a loss of one of the alleles. but how does MLPA work? anyone know any good sites that explain it ?

MLPA is = Multiplex Ligation-dependent Probe Amplification (MLPA) - high resolution method to detect copy number variation in genomic sequences.

but how would microsatellite markers and MLPA be able to tell you whether its a LOH or a aUPD ? basically whether you have got a REAL loss of one of the chromosomes, or a duplication of the SAME chromosome.
THANK you so much in advance!!! I REALLY appreciate it !!!

#2 Lala

Lala

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 07 October 2009 - 05:51 AM

i cant edit my original post.

so just wanted to say. i know what the A stands for, which is ACQUIRED uniparental disomy.

still working on how to tell the difference between LOH and aUPD <_<




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.