2 replies to this topic

[novagen]

Hi all,

How to predict exons,introns,utrs and splice sites in a plant genomic DNa sequence.

Thanks all

[gleb.kudr]

3' bound of intron are often marked with GTA (and sometimes GT*)

5' bound are: CAG, but another TAG or CGG are also present.

And use blast algorithm to predict place of introns between close related species.

There is nothing other marks in sequence to predict nuclear intrones (group 1 or 2 is another case and search for literature for it's prediction)

Edited by gleb.kudr, 07 October 2009 - 03:04 AM.

[novagen]

gleb.kudr, on Oct 7 2009, 04:03 AM, said:

3' bound of intron are often marked with GTA (and sometimes GT*)

5' bound are: CAG, but another TAG or CGG are also present.

And use blast algorithm to predict place of introns between close related species.

There is nothing other marks in sequence to predict nuclear intrones (group 1 or 2 is another case and search for literature for it's prediction)

Thanx a lot for your reply