RNA Extraction Mice Adipose Tissue
Posted 06 October 2009 - 07:34 AM
I just wanted to know what's your protocol for extracting this kind of tissue? I use Trizol with an extra chloroform extraction. I haven't gotten good results from this and have never gotten an RNA pellet. Though I don't get an RNA pellet I still continue until the end and most of the time get a 260:280 ratio above 2.1 (usually around 4-6). I tested for contamination, but I always get good results for other tissue (i.e. liver). I'm just having a hard time on this one. If you can help me thanks!
Posted 06 October 2009 - 08:05 AM
then heaven will be yours, before you meet your end
Posted 06 October 2009 - 08:20 AM