PBS or TBS
Posted 05 October 2009 - 08:43 AM
I'm having some problems with my western for a phosphorylated protein, the first time I did it on some old samples to test the antibody using PBS (by mistake) it worked better than it is now when using the TBS.... Do you always have to use TBS for a phospho antibody?? or is it just sometimes better? I'm down to my last bit of protein and still struggeling to get a band I can do densitometry on.. any ideas?
Posted 05 October 2009 - 09:22 AM
Ultimately, do what works for you and your western.
Posted 05 October 2009 - 03:36 PM
Posted 06 October 2009 - 05:50 PM
Edited by miBunny, 06 October 2009 - 05:51 PM.
Posted 07 October 2009 - 12:46 AM