Plasmids for protein expression in mammalian cells
Posted 01 October 2009 - 04:08 PM
I have constructs for fragments and whole protein.
I use TNT lysate (Promega) to express proteins to test protein interactions.
I've tried different plasimds but the results were not good.
Can someone give me advice or some information about what plasmids are best for protein expression in lysate ( mammalian cells)?
Also what are the features that should be in plasmids to use them for protein expressin?
Thank you in advance
Posted 01 October 2009 - 05:21 PM
Have you thought of using other lysates instead of the promega one that you are using currently?
Posted 01 October 2009 - 06:01 PM
In fact we just used Promega products. Sorry, why you suggest using another lysate?