Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Native PAGE w. Western protein marker


  • Please log in to reply
4 replies to this topic

#1 katenkak

katenkak

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 52 posts
0
Neutral

Posted 01 October 2009 - 06:56 AM

Hi all!
I'd like to run a native PAGE followed by Western. What native protein standard I can use for that? I need it to be stained, but I can't find anything on a net. Does anybody knows something? Thanks for advice!

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,830 posts
414
Excellent

Posted 01 October 2009 - 03:33 PM

Try NativeMark from invitrogen

#3 katenkak

katenkak

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 52 posts
0
Neutral

Posted 02 October 2009 - 12:31 AM

Hi! I saw this marker, but it is NOT stained! I need something I could blot on the membrane along with my protein of interest and see the marker on membrane as well.

Try NativeMark from invitrogen



#4 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,811 posts
136
Excellent

Posted 02 October 2009 - 10:35 AM

prestained markers are denatured (i'm not aware of any prestained native gel standards). if you want native markers then you will have to transfer and stain the membrane with ponceau s to see them.

Edited by mdfenko, 02 October 2009 - 10:39 AM.

talent does what it can
genius does what it must
i do what i get paid to do

#5 katenkak

katenkak

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 52 posts
0
Neutral

Posted 03 October 2009 - 11:20 AM

Thank you. This is confirmation of what I was suspecting.

prestained markers are denatured (i'm not aware of any prestained native gel standards). if you want native markers then you will have to transfer and stain the membrane with ponceau s to see them.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.