Hi all!
I'd like to run a native PAGE followed by Western. What native protein standard I can use for that? I need it to be stained, but I can't find anything on a net. Does anybody knows something? Thanks for advice!
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#2
bob1
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Posted 01 October 2009 - 03:33 PM
Try NativeMark from invitrogen
#3
katenkak
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Posted 02 October 2009 - 12:31 AM
Hi! I saw this marker, but it is NOT stained! I need something I could blot on the membrane along with my protein of interest and see the marker on membrane as well.
bob1, on Oct 2 2009, 01:33 AM, said:
Try NativeMark from invitrogen
#4
mdfenko
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Posted 02 October 2009 - 10:35 AM
prestained markers are denatured (i'm not aware of any prestained native gel standards). if you want native markers then you will have to transfer and stain the membrane with ponceau s to see them.
Edited by mdfenko, 02 October 2009 - 10:39 AM.
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genius does what it must
i do what i get paid to do
#5
katenkak
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Posted 03 October 2009 - 11:20 AM
Thank you. This is confirmation of what I was suspecting.
mdfenko, on Oct 2 2009, 08:35 PM, said:
prestained markers are denatured (i'm not aware of any prestained native gel standards). if you want native markers then you will have to transfer and stain the membrane with ponceau s to see them.
