Hi!
I am currently making an extraction buffer for plant ground tissue containing sodium citrate 50 mM pH8.3, 20 mM DIECA and 2% polyvinylpirrolidone.
I see that polyvinylpirrolidone or something in my buffer seems not to have dissolved very well. Do you know if there is any particular way to dissolve it? should I warm it in the microwave oven? Should I filtrate it for sterilization? Any ideas?
I sincerely have no idea, it is the first time I do this.
Thanks!
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3 replies to this topic
#2
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an elder
Posted 02 October 2009 - 08:22 AM
you could try warming it on a hot plate stirrer.
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genius does what it must
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genius does what it must
i do what i get paid to do
#3
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member
Posted 04 October 2009 - 07:56 PM
hi
I used to work with PVP 40 sometime back for DNA extraction. I used 55 C water bath to 5- 10 min to dissolve it in the buffer. This should work but pls check the temperature in a catologue in case.
good luck
chandima
I used to work with PVP 40 sometime back for DNA extraction. I used 55 C water bath to 5- 10 min to dissolve it in the buffer. This should work but pls check the temperature in a catologue in case.
good luck
chandima
