How to dissolve polyvinylpirrolidone (PVP K25)
Posted 01 October 2009 - 05:27 AM
I am currently making an extraction buffer for plant ground tissue containing sodium citrate 50 mM pH8.3, 20 mM DIECA and 2% polyvinylpirrolidone.
I see that polyvinylpirrolidone or something in my buffer seems not to have dissolved very well. Do you know if there is any particular way to dissolve it? should I warm it in the microwave oven? Should I filtrate it for sterilization? Any ideas?
I sincerely have no idea, it is the first time I do this.
Posted 02 October 2009 - 08:22 AM
genius does what it must
i do what i get paid to do
Posted 04 October 2009 - 07:56 PM
I used to work with PVP 40 sometime back for DNA extraction. I used 55 C water bath to 5- 10 min to dissolve it in the buffer. This should work but pls check the temperature in a catologue in case.