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[bethh]

I am currently trying to produce a stable cell line that is cotranfected with the expression plasmid and a selection plasmid. So far despite doing single cell dilutions and using cloning rings have I been able to get a pure population.

Any suggestions on a better way to do this? (Can I label both plasmids and then FACs sort them?) I'm tired of doing lots of patch clamp experiments just to determine if we have a good population.

Any tips?