Hi
I purified RNA on PAA denaturating gel and after this I stained gel with stains all. I cut out the band with my RNA and tried to eluted it with 0,3 M sodium acetate. I found that I couldn't elute RNA. Does anybody know where is the problem? Maybe the stains all affects elution? From the other hand I eluted the same RNA without staining and everything was fine.
cheers
Leszek
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3 replies to this topic
#2
bob1
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Thelymitra pulchella
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Posted 30 September 2009 - 03:26 PM
Which stain did you use? some will cause the gel to cross-link the RNA.
#3
blaszcz
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Posted 01 October 2009 - 04:50 AM
I use Stains-All from sigma http://www.sigmaaldr...c...S=ON&F=SPEC
#4
mdfenko
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an elder
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Posted 02 October 2009 - 08:16 AM
in what medium do you prepare the stains-all?
we used it for protein staining and prepared it in a solution containing isopropanol. if you prepared yours in a similar solution then you may have precipitated the rna in the gel and may require longer incubation and/or harsher conditions to elute.
we used it for protein staining and prepared it in a solution containing isopropanol. if you prepared yours in a similar solution then you may have precipitated the rna in the gel and may require longer incubation and/or harsher conditions to elute.
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