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RNA elution after stains all staining


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#1 blaszcz

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Posted 30 September 2009 - 07:18 AM

Hi

I purified RNA on PAA denaturating gel and after this I stained gel with stains all. I cut out the band with my RNA and tried to eluted it with 0,3 M sodium acetate. I found that I couldn't elute RNA. Does anybody know where is the problem? Maybe the stains all affects elution? From the other hand I eluted the same RNA without staining and everything was fine.

cheers

Leszek

#2 bob1

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Posted 30 September 2009 - 03:26 PM

Which stain did you use? some will cause the gel to cross-link the RNA.

#3 blaszcz

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Posted 01 October 2009 - 04:50 AM

I use Stains-All from sigma http://www.sigmaaldr...c...S=ON&F=SPEC

#4 mdfenko

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Posted 02 October 2009 - 08:16 AM

in what medium do you prepare the stains-all?

we used it for protein staining and prepared it in a solution containing isopropanol. if you prepared yours in a similar solution then you may have precipitated the rna in the gel and may require longer incubation and/or harsher conditions to elute.
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