RNA elution after stains all staining
Posted 30 September 2009 - 07:18 AM
I purified RNA on PAA denaturating gel and after this I stained gel with stains all. I cut out the band with my RNA and tried to eluted it with 0,3 M sodium acetate. I found that I couldn't elute RNA. Does anybody know where is the problem? Maybe the stains all affects elution? From the other hand I eluted the same RNA without staining and everything was fine.
Posted 30 September 2009 - 03:26 PM
Posted 02 October 2009 - 08:16 AM
we used it for protein staining and prepared it in a solution containing isopropanol. if you prepared yours in a similar solution then you may have precipitated the rna in the gel and may require longer incubation and/or harsher conditions to elute.
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