Hi,
I am working on two dimensional gel electrophoresis-based proteomics on a non-model species (i.e. genome not sequenced).
Recently, a team generated the cDNA library of my studied species. May I know if I can use these EST information to help identifiy the proteins that could not be matched after MALDI TOF/TOF?
Thank you, appreciated.
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3 replies to this topic
#2
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Hmmm, I think it's working
Posted 30 September 2009 - 03:45 PM
Sure, there shouldn't be a problem with it, be aware that RNA doesn't always equal protein though.
#3
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Guitar Hero
Posted 01 October 2009 - 07:09 AM
i think you should consider codon usage as well. by doing a blastx you should be able to find it tho
"When there's no more room in hell the dead will walk the Earth"
#4
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Enthusiast
Posted 01 October 2009 - 09:05 PM
bob1, on Oct 1 2009, 07:45 AM, said:
Sure, there shouldn't be a problem with it, be aware that RNA doesn't always equal protein though.
Hi, bob1
May I know what program should I use in order to carry out the search?
Thx again.
